Supplementary Figure 6: Germline mutations in met-2 set-25 worms.

Further verification and characterization of the mutations detected in the genome sequencing experiment described in Figure 6. (a) Number and distribution of single-nucleotide variants (SNVs) or polymorphisms observed in the sequencing experiment described in Figure 6a. No dinucleotide preferences were found among SNVs. (b) Sketch of a complex rearrangement involving a Tc3 transposon, found exclusively in the met-2 set-25 genome. The rearrangement was identified by genome sequencing. The graph to the left indicates the precise site of Tc3 insertion. Below are H3K9me2 and H3K9me3 ChIP-seq tracks for the region around the Tc3 transposon that provoked the inversion: it bears high levels of H3K9me3 and showed roughly a twofold change in expression in met-2 set-25 over wt gonads at 20 °C. (c) Southern blotting with a probe against Tc3 shows a novel band detected in the met-2 set-25 mutant. (d) Depiction of the rearrangement shown in b indicating the position of the primer pairs used to verify the rearranged genomic context by PCR. PCR confirmation of the rearrangement is shown to the right. (e) Copy number ratios of met-2 set-25 worms relative to wt for the entire telomeric repeat subfamily, and for single telomere repeats. (f) Copy number ratios of met-2 set-25 worms relative to wt for rDNA repeats.