Supplementary Figure 1: Immunofluorescence (IF) confirms absence of H3K9me in met-2 set-25 worms.

IF images of wild-type (wt) and met-2 set-25 worms showing the loss of H3K9me2/me3 at the indicated developmental stages grown at 20 °C (Online Methods). The staining of H4 pan-acetyl (H4ac) served as a positive control. (a) Gonads (bar, 20 μm). (b) Embryos and L2-stage larvae (bar, 20 μm).