Supplementary Figure 6: Reconstruction of phyloepigenetic trees with four different probe selection cutoffs (0.2, 0.3, 0.4 and 0.5).

The probe selection cutoff defines the minimum delta methylation level (β value) used for variable selection (‘shared’ versus ‘private’ status) to choose the methylation probes to be used in construction of trees. Each row shows one case, and each column shows the resulting tree for each cutoff. The cutoff used in the results section (Fig. 4a) was 0.3. The number of probes selected (n) and Robinson-Foulds (RF) distance to the original tree (cutoff = 0.3) are provided for each tree (except for the one with cutoff = 0.3, which is the reference). ESCC05 did not have enough probes to construct a tree at cutoff = 0.5.