Supplementary Figure 7: Characterization of SH3PXD2A-HTRA1 region on chromosome 10 from exome sequencing coverage.

(a) Zoomed-out view of chromosome 10. The dotted red vertical line on the left is chr10:105,452,000 (SH3PXD2A area) and the dotted red line on the right is chr10: 124,248,418 (HTRA1). Ample DNA exome coverage of intervening sequences in a fusion-positive sample and comparable exome coverage to matched blood and a fusion-negative patient suggests lack of large-scale genomic rearrangement to generate this fusion. (b,c) Zoomed-in image of aligned DNA reads around fusion breakpoint (vertical red line) for SH3PXD2A and HTRA1. No clear loss of read depth at exons involved at the fusion junction suggests that the rearrangement may occur in an intronic region or another genomic location.