Supplementary Figure 14: Nuclear localization and transactivation activity of ERGalt.
From: Deregulation of DUX4 and ERG in acute lymphoblastic leukemia
(a) Immunofluorescence of Arf–/– pre-B cells transduced with retroviral vectors with no ERG construct (MIG), wild-type ERG (MIG-ERG WT) or ERGalt (MIG-ERG e6alt) showing nuclear localization of ERG. Scale bars, 10 μm. (b) Competition assay to determine the transcriptional activity of wild-type (WT) ERG and ERGalt (MT) supplemented with empty vector (MIG) determined using the pGL3-gpIX luciferase reporter in HEK293T cells. Bars show means ± s.e.m. luciferase activity was derived from two individual experiments with triplicate measurements. (c) Immunoblotting of HEK293T cells transfected with empty vector or ERG plasmids confirming the expression shown in b.
