Supplementary Figure 7: Tumor progression of transplanted IRS4-overexpressing HCC-15 cells and mock control in mouse model. | Nature Genetics

Supplementary Figure 7: Tumor progression of transplanted IRS4-overexpressing HCC-15 cells and mock control in mouse model.

From: Pan-cancer analysis of somatic copy-number alterations implicates IRS4 and IGF2 in enhancer hijacking

Supplementary Figure 7

Related to main Figure 3. (a) Boxplots depicting mouse tumor progression curves of HCC-15 cells containing IRS4-expressing lentiviral constructs (pLenti-IRS4) versus mock control (pLenti-empty HCC-15); 1x106 cells injected respectively; last time point: 1st P=0.046, 2nd P=0.03; two-tailed t-test; two-tailed t-test computed at last measured time point (day 39); N=8 for each group in first experiment, N=9 for control and N=12 for IRS4 overexpressing sample in second experiment. (b) RT-qPCR of the control HCC-15 and IRS4-HCC-15 tumors confirming IRS4 overexpression. (c) Flow cytometry of tumors injected with IRS4-IRES-GFP overexpressing vector HCC-15 cell line. (d) Representative immunohistochemistry experiment showing IRS4 overexpression in the harvested tumors (bottom panel) but not in the control tumors (top panel).

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