Supplementary Figure 10: Absence of GMP progenitors in Smarcd2^−/− fetal liver hematopoiesis.

(a–c) Representative example of wild-type (a) and knockout (b) fetal liver hematopoiesis (per embryo) and gating strategy (c). Cells were first discriminated by FSC/SSC (data not shown) and then by SSC-H/CD45. CD45⁺ cells were gated on Lin⁻c-Kit⁺ cells. CD45⁺Lin⁻ Sca-1⁺c-Kit⁺ (LSK) cells were sorted, and CD45⁺Lin⁻CD117⁺Sca-1⁻ (myeloid progenitor) cells were further separated into CD45⁺Lin⁻Sca-1⁻c-Kit⁺CD34⁻CD16/32^lo MEPs, CD45⁺Lin⁻Sca-1⁻c-Kit⁺CD34⁺CD16/32^int CMPs, and CD45⁺Lin⁻Sca-1⁻c-Kit⁺CD34⁺CD16/32^hi GMPs. In comparison to a, GMP cells are almost absent in b. (c) Numbers in black boxes refer to the percentage of wild-type cells, and numbers in white boxes refer to the percentage of knockout cells.