Supplementary Figure 12: Immunoprecipitation of endogenous CEBPɛ.

(a–d) Experiment 1. (a) Immunoblotting for SMARCD2 detects SMARCD2 in a CEBPɛ immunoprecipitate in short and long exposures; a distinct band is noted (red arrow) at the expected size of ~56 kDa, corresponding to input and coimmunoprecipitated SMARCD2 proteins. (b) Cropped lanes of a showing immunoprecipitation with isotype control and antibody to CEBPɛ (a2). (c) CEBPɛ is detected in input and immunoprecipitate; the image was cropped (c3) for summary. (d) Cropped images highlighting bands from experiment 1 shown with standard kDa marker (PageRuler Prestained Protein Ladder, Thermo Fisher). (e–g) Experiment 2. (e) Immunoblotting for SMARCD2 detects SMARCD2 in two independent CEBPɛ immunoprecipitates from the same lysate (technical replicates). Distinct bands are noted (red arrows) at the expected size of ~56 kDa, corresponding to input and coimmunoprecipitated SMARCD2 proteins (e1). Immunoblotting for GAPDH shows the presence of GAPDH in input but not in immunoprecipitates (e3). (f) CEBPɛ is detected in input and immunoprecipitates (red arrows) (f2). (g) Cropped images highlighting bands from experiment 2 shown with standard kDa marker (PageRuler Prestained Protein Ladder, Thermo Fisher). (h–j) Experiment 3. (h) Immunoblotting for SMARCD2 detects SMARCD2 in the CEBPɛ immunoprecipitate. A distinct band is noted (red arrow) at the expected size of ~56 kDa, corresponding to input and coimmunoprecipitated SMARCD2 (h1). Immunoblotting for GAPDH shows the presence of GAPDH in Input but not in immunoprecipitates (red arrow) (h3). (i) CEBPɛ is detected in input and immunoprecipitates (red arrows) (i2). (j) Cropped images highlighting bands from experiment 3 shown with standard kDa marker (Precision Plus Protein Kaleidoscope Prestained Protein Standards, Bio-Rad). The protein standard ladders differ between experiment 1 and 2 and experiment 3.