Figure 5: Candidate genes associated with citrus polyembryony.
From: Genomic analyses of primitive, wild and cultivated citrus provide insights into asexual reproduction
(a) Beeswarm and box plot for associated SNPs in the 11 candidate genes. The dots in different colors represent associated SNPs in the corresponding gene sequence. Boxes, median and interquartile range; whiskers maximum and minimum values. (b) Relative expression of CitRWP as determined by qRT-PCR in different tissues of sweet orange. Data are mean ± s.d.; n = 3 technical replicates of 2 pooled tissue samples. (c) qRT-PCR validation of CitRWP expression in the ovules of monoembryonic cultivars (PML, pummelo; CTR, citron; CLM, clementine mandarin) and polyembryonic cultivars (GRP, grapefruit; LM, lemon; PK, ponkan). Data are mean ± s.d.; n = 3 technical replicates of 2 pooled tissue samples. (d) Schematic diagram of CitRWP. Asterisks indicate positions of SNPs associated with polyembryony; triangle indicates MITE insertion; orange rectangles indicate exons. (e) Representative PCR-based detection of MITE insertions in citrus cultivars. (f) PCR-based analysis of the MITE insertion in 786 citrus accessions and the F1 segregating population derived from the HB pummelo (HB) × Fairchild mandarin (FC) cross.
