Figure 6: Comparative transcriptome analysis of monoembryonic and polyembryonic citrus.
From: Genomic analyses of primitive, wild and cultivated citrus provide insights into asexual reproduction
(a) Heat map of genes that are specifically expressed in ovules of both pummelo (monoembryony) and sweet orange (polyembryony). Leaf, ovule, fruit and seed tissues from pummelo (odd numbers) and sweet orange (even numbers) were used for the comparative analysis. (b) Heat map of the genes with significantly different expression in the ovules of monoembryonic (PML, pummelo; CTR, citron; CLM, clementine mandarin) and polyembryonic cultivars (GRP, grapefruit; LM, lemon; PK, ponkan). Data are derived from 2 replicates of plant tissue. Normalized FPKM values after scaling and centering are shown. (c) Model for genes that are involved in monoembryony and polyembryony, including the candidate gene CitRWP (yellow star). Blue dots indicate genes highly expressed in ovules and showed no significant difference in expression between monoembryonic and polyembryonic citrus cultivars (Supplementary Tables 36 and 37). Red dots indicate genes highly expressed in the ovules of polyembryonic citrus cultivars. M, megaspore mother cell; ES, embryo sac; N, nucellar embryo initiation cell.
