Supplementary Figure 2: Fab2L epiline establishment.
From: Stable Polycomb-dependent transgenerational inheritance of chromatin states in Drosophila
A- Phenotypic classification based on eye pigment levels in Fab2LWhite*, Fab2L and Fab2LRed* female flies (n>120). Class 1: pigment=0%; Class 2: 0%<pigment≤5%; Class 3: 5%<pigment≤75%; Class 4: 75%<pigment<100%; Class 5: pigment=100%.
B- Crossing schemes for phenotypic selection and charts representing the percentage of Class1 (pigment=0%) male flies in grey and Class 5 (pigment=100%) male flies in red, before (P0) and after (F12) the phenotypic selection (n>40). Note that the presence of the TM6 balancer in the F1, used here as a control, did not lead to establishment of any epiallele.
Bars represent the frequency of the flies scored; two-tailed Fisher’s exact test: NS P>0.05; * P<0.05; ** P<0.01; *** P<0.001.
