Supplementary Figure 6: 37B- and 89E-loci long-range chromatin interactions and homologous unpairing in Fab2L lines.
From: Stable Polycomb-dependent transgenerational inheritance of chromatin states in Drosophila
A,B- Box plots representing the distance distributions of the FISH assays performed in the indicated genotypes between the 37B (transgene insertion locus) and the 89E (endogenous Fab-7 locus) loci. Distances are measured in stage 14-15 embryos in T1 and T2 segments or in the germline. The centerline represents the median, the box delimits the interquartile-range and the limits define the distribution range. n represents the total number of nuclei analyzed from 3 embryos.
C,D- Charts representing the distance distributions of the FISH assays performed in the indicated genotypes between the 37B and the 89E loci. Distances are measured in stage 14-15 embryos in T1 and T2 segments or in the germline.
E- Frequency of homologous unpairing at the 37B and the 89E loci in the FISH assays performed in the indicated genotypes. Levels of unpairing are measured in stage 14-15 embryos in T1 and T2 segments, considering a minimum threshold distance between homologous loci of 0.5 μm.
Bars represent the frequency of distances between 37B and 89E loci (C,D) or the frequency of unpairing at 37B and 89E loci (E). In the figure, n represents the total number of nuclei analyzed from 3 embryos; two-tailed Student’s t-test (A-D) or two-tailed Fisher’s exact test (E); NS P>0.05; * P<0.05; ** P<0.01; *** P<0.001.
