Supplementary Figure 8: Effects of the number of Fab-7 loci and of the presence of endogenous Mcp on epiallele maintenance.

A- Crossing schemes, eye pigmentation assays and cartoons of the experiment testing the impact of Fab-7 copy number on epiallele maintenance. The pictures are representative of the observed phenotypes. In the cartoons, the green chromosomes represent chromosomes X (acrocentric) and Y (metacentric) chromosomes, the blue chromosomes represent chromosome 2, the red chromosomes represent chromosome 3, the black lines represent the transgenic insertion on chromosome X and/or 2 or the endogenous Fab-7 on chromosome 3, the white triangle represent the deletion of the endogenous Fab-7 and the asterisks indicate the presence of the epiallele. On the right, the counting of total number of Fab-7 copies, of endogenous Fab-7 copies and the presence or not of the epiallele for each condition.

B- Crossing schemes, eye pigmentation assays and representative pictures of the phenotypes observed in the Mcp[1] epiallele maintenance tests. The single crosses in the F2 have been performed in order to unambiguously distinguish between hemizygous and homozygous Mcp[1] males. Pictures represent wt and Mcp[1] male flies with an A4 to A5 homeotic transformation (yellow arrows), carrying either Fab2L^White* or Fab2L^Red* epiallele.

Bars represent the mean of n=3 adult head collections, coming from the same original cross +/- s. d.; two-tailed Student’s t-test: NS P>0.05; * P<0.05; ** P<0.01; *** P<0.001.