Supplementary Figure 11: Chromatin-mark deposition at the transgenic locus in embryos.
From: Stable Polycomb-dependent transgenerational inheritance of chromatin states in Drosophila
A- ChIP-qPCR assays performed on w[1118], Fab2LWhite*, Fab2L and Fab2LRed* embryos 8 to 12 hours showing relative enrichments (ChIP/Input) for H3K27me3 normalized to a negative control. Amplicon locations are indicated below the charts.
B-D- ChIP-qPCR assays performed on w[1118], Fab2LWhite*, Fab2L and Fab2LRed* embryos 4 to 8 hours, showing relative enrichments (ChIP/Input) for H3K4me3, H3K9/K14ac and H4panacetylated normalized to a negative control. Amplicon locations are indicated below the charts.
Bars represent the mean of n=3 independent embryo collections +/- s. d.; two-tailed Student’s t-test: NS P>0.05; * P<0.05; ** P<0.01; *** P<0.001.
