Supplementary Figure 12: 37B-locus colocalization with Polycomb foci in the epilines.
From: Stable Polycomb-dependent transgenerational inheritance of chromatin states in Drosophila
A- The charts show the percentage of centers of mass of the FISH signals (37B locus) that colocalize with a Polycomb focus in the indicated lines. In the figure, n represents the total number of FISH signals analyzed from 4 embryos. FISH-I assays were performed in T1 and T2 segments of stages 14-15 embryos.
B- The box plots show the distributions of the relative intensity of Polycomb within the centers of mass of the FISH signals for the different lines. In the figure, n represents the total number of Polycomb foci analyzed from 4 embryos. Polycomb intensities were scored only when FISH signals (37B locus) colocalized with Polycomb. The centerline represents the median, the box delimits the interquartile-range, the limits define the distribution range and the dots represent the outliers.
C- Examples of the FISH-I assays performed in the indicated epilines. Nuclei are stained with DAPI in blue, 37B locus in red, Polycomb in green. Scale bar is 1 μm.
Bars represent the frequency of colocalization (A); two-tailed Fisher’s exact test (A) or two-tailed Mann-Whitney test (B): NS P>0.05; * P<0.05; ** P<0.01; *** P<0.001.
