Supplementary Figure 7: 6mA presence or absence is related to gene function.
From: Widespread adenine N6-methylation of active genes in fungi
a) Methylation presence/absence at all genes containing common pfam17 domains (present in at least 8 genes) and their deviation from expected. Pfams showing significant (p ≤ 0.05) departures from the expected were identified using Fisher’s exact test followed by FDR correction (significant = red, non-significant = blue). Overall percentage of significant pfams for each genome are shown in parenthesis next to lineage names. b) log2 fold change in methylation presence/absence at genes containing common pfam17 domains across all lineages (present in at least 8 genes across all genomes, significant in at least one lineage). Lineages showing significant departure from the expected are denoted with a * (adjusted p-value ≤ 0.05), or ** (adjusted p-value ≤ 0.01). Green = enriched in unmethylated gene set, purple = enriched in methylated gene set. Constitutively expressed housekeeping proteins, such as mitochondrial Rho proteins (blue arrow) are very frequently methylated, while some genes, such as Leucine-Rich-Repeat containing proteins (orange arrow) show variability across lineages.
