Supplementary Figure 2: Validation of SMRT-detected 6mA using mass spectrometry and IP sequencing.
From: Widespread adenine N6-methylation of active genes in fungi
a) Percent methylated adenines as detected by SMRT-analysis (SMRT-6mA) and by Mass-Spectrometry (MS-6mA). As a measure of high confidence SMRT-detected sites, percent of methylated adenines at ApT sites (SMRT-6mApT) is also included. b) 6mA overlap between SMRT and IP-seq methods within a 100kb region of H. vesiculosa scaffold 1. Red tiles: MACs detected through SMRT-analysis. Black tiles: methylated regions identified through IP-sequencing. Significant peaks were detected using macs231, Q-value ≤ 0.01. Read coverage tracks for both the control (inner circle) and pulldown (outer circle) are also shown. c) Comparison of 6mA-IP and SMRT analysis results across all lineages examined. a refers to percent of 6mA bases identified by SMRT analysis prior to filtering.
