Supplementary Figure 9: DZIP1L specifically associates with the ciliary-transition-zone protein SEPT2. | Nature Genetics

Supplementary Figure 9: DZIP1L specifically associates with the ciliary-transition-zone protein SEPT2.

From: Mutations in DZIP1L, which encodes a ciliary-transition-zone protein, cause autosomal recessive polycystic kidney disease

Supplementary Figure 9

(a) Co-IP experiments from HEK293T cells transfected with expression constructs for epitope-tagged SEPT2 (C-terminal HA) and DZIP1L, CEP89, B9D1, MKS1 and NPHP1 (N-terminal Myc), reveal a specific interaction between SEPT2 and DZIP1L but none of the other proteins analysed. (b) Co-IP with DZIP1L (C-terminal HA) and SEPT2, CEP89, B9D1, MKS1 and NPHP1 (N-terminal Myc) confirmed the DZIP1L-SEPT2 interaction, and showed that none of the other proteins analysed interact with DZIP1L. Co-IP experiments were repeated three times.

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