Supplementary Figure 9: PRRX1 is expressed in neuroblastoma cells.

(a) Representative false-color image of double-IHC for MYCN (blue) and PRRX1 (red). MYCN⁺PRRX1⁺ cells are shown in yellow. Scale bar, 100 μm. (b) Percentage of MYCN–PRRX1 double-positive cells relative to all PRRX1⁺ cells in six stage 4 neuroblastoma tumors. (c) Dot plot visualization of MAML3 mRNA expression levels (Affymetrix probeset 242794_at) in three independent neuroblastoma series (red) as compared to eight other tumor types (blue) and nine normal tissues (green). (d) Hematoxylin and eosin staining (HE; left) and double-IHC for MAML3 (blue, nuclear) and the ADRN markers DBH (middle; red, cytoplasmic) and TH (right; red, cytoplasmic) on serial neuroblastoma sections from a representative stage 4 neuroblastoma. Str, Schwannian stroma; Nbl, neuroblasts with small round blue morphology. Scale bar, 100 μm.