Supplementary Figure 7: Additional details on the insertion patterns and differential expression of the candidate genes.

(a) Insertion pattern in Gab1, showing bias toward activating insertions. (b) Boxplots for the main candidate genes showing the difference in expression after the insertion sites in the corresponding gene between samples with and without an insertion. The boxplots and P values were calculated using IM-Fusion’s differential expression test, which essentially compares the expression of exons after the insertion sites in each gene between samples with an insertion and samples without an insertion, after normalizing for differences in overall expression between samples (see the Online Methods for more details). Boxes extend from the third (Q3) to the first (Q1) quartile (IQR), with the line at the median; whiskers extend to Q3 + 1.5 IQR and Q1 − 1.5 IQR. P values were calculated using the non-parametric Mann–Whitney U test. Green/purple boxplots indicate significant increases and decreases in expression (P < 0.05), respectively. (c) Insertion pattern in Trps1, showing bias toward truncating/inactivating insertions.