Supplementary Figure 13: Overview of Cre-conditional alleles and distribution of the tumor morphology in the different genetically engineered mouse models. | Nature Genetics

Supplementary Figure 13: Overview of Cre-conditional alleles and distribution of the tumor morphology in the different genetically engineered mouse models.

From: Insertional mutagenesis identifies drivers of a novel oncogenic pathway in invasive lobular breast carcinoma

Supplementary Figure 13

(a) Depiction of Cre-conditional invCAG-Ppp1r12aex1-9-IRES-Luc and invCAG-Trp53bp2ex13-18-IRES-Luc alleles in the Col1a1 locus. Cre-mediated recombination allows inversion of the CAG promoter, resulting in expression of PPP1R12A1-418aa or TRP53BP2766-1134aa accompanied by luciferase expression. (b) Recombination status of the Cre-conditional alleles in tumors from Wap-Cre;Cdh1F/F;Ppp1r12 aex1-9 and Wap-Cre;Cdh1F/F;Trp53bp2ex13-18 mice, as visualized by PCR. EcadF and EcadD are PCRs to detect the Cdh1F and Cdh1Δ alleles, respectively. ShuttleR detects the recombined (p1 and p3; 1,054 bp) and non-recombined (p1 and p2; 897 bp) Cre-conditional alleles (primer positions are shown in a). (c) Histological classification of tumors from Wap-Cre;Cdh1F/F;Ppp1r12aex1-9 (n = 27) and Wap-Cre;Cdh1F/F;Trp53bp2ex13-18 (n = 30) mice. (d) Histological classification of tumors (n = 11) from Wap-Cre;Cdh1F/F;Cas9 mice injected with Lenti-CRISPR-sgMyh9.

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