Supplementary Figure 8: ARHGEF26 promoter activity luciferase assay.

The −2,516 to +2 region 5′ of the ARHGEF26 gene was cloned for the haplotypes with the G (reference) and C (alternative) alleles at rs12493885. The reference and alternative haplotypes were coupled with a firefly luciferase reporter gene and cotransfected with a Renilla luciferase co-reporter in HEK293 cells, HAECs, and HUVECs. Promoterless firefly luciferase reporter was included as negative control. Firefly luciferase activity relative to Renilla luciferase activity was measured 48 h after transfection and is expressed as fold changes over promoterless vectors (HEK293 cells, n = 4; HAECs, n = 6; HUVECs, n = 6; mean ± s.d.; separate one-way ANOVA with Tukey’s multiple-comparisons tests and multiplicity-adjusted P values for each cell type; F = 23.88, DF = 2 for HEK293 cells; F = 0.8038, DF = 2 in HAECs; F = 0.02397, DF = 2 in HUVECs).