Supplementary Figure 9: Western blots of transfected vascular cells. | Nature Genetics

Supplementary Figure 9: Western blots of transfected vascular cells.

From: Genetic analysis in UK Biobank links insulin resistance and transendothelial migration pathways to coronary artery disease

Supplementary Figure 9

HAECs or HCASMCs were transfected with non-targeting siRNA plus empty vector (Control), siRNA against ARHGEF26 3′ UTR and empty vector (siRNA + empty vector), siRNA and a wild-type FLAG-ARHGEF26 vector (siRNA + WT), or siRNA and a mutant vector (siRNA + Leu29). Transfected HAECs or HCASMCs were harvested 72 h after transfection. Normalized cell lysates (20 μg/lane) were resolved by SDS–PAGE and probed for ARHGEF26, FLAG, and actin by respective antibodies and imaged by enhanced chemiluminescence.

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