Supplementary Figure 11: Evaluation of ARHGEF26 protein stability in cells.

Wild-type (WT) or Leu29 Flag-ARHGEF26 were overexpressed in HEK293 cells for 48 h followed by treatment with 50 μg/mL and 100 μg/mL cycloheximide. Cells were harvested at indicated time points after treatment, and normalized lysates (20 μg/lane) were probed for FLAG by western blot. For each cycloheximide dose, two blot sections (WT and Leu29) from the same membrane simultaneously imaged are shown in juxtaposition for contrast.