Supplementary Figure 6: Exogenous expression of Prdm15 partially substitutes for 2i and supports naive pluripotency.
From: PRDM15 safeguards naive pluripotency by transcriptionally regulating WNT and MAPK–ERK signaling
(a) Differentially expressed genes in wild-type (EV) versus PRDM15-overexpressing ESCs, associated with primed or naive pluripotency. (b) mRNA levels of naive (Tbx3 and Prdm14) and primed (Otx2, Fgf5 and Krt18) markers in wild-type (EV) versus PRDM15-overexpressing ESCs. Cells were cultured in medium containing two (2i), one (Meki or CHIR) or zero (N2B27) inhibitors. Expression levels are normalized to those of Ubiquitin and presented as fold change compared to wild-type cells under the 2i condition. One representative experiment is shown (n = 2); data are from three independent cultures; center values, mean; error bars, s.d. Student’s t test (two-sided) was used.
