Supplementary Figure 10: Restoration of naive pluripotency in Prdm15Δ/Δ ESCs by rescue of target gene expression.
From: PRDM15 safeguards naive pluripotency by transcriptionally regulating WNT and MAPK–ERK signaling
(a) Immunofluorescence staining of Prdm15fl/fl versus Prdm15Δ/Δ ESCs in SL using antibodies against β-CAT (green) and CDH1 (red). DNA was stained with DAPI (blue); cells were cultured either in untreated (n.t.) or RSPO1 conditioned medium (CM). (b) Immunofluorescence staining of TBX3 (green) and NANOG (red) in Prdm15fl/fl, Prdm15Δ/Δ, Prdm15Δ/Δ; SPRY1-OE and Prdm15Δ/Δ; RSPO1-OE ESCs. (c) Immunofluorescence staining of TBX3 (green) and NANOG (red) in Prdm15fl/fl, Prdm15Δ/Δ and Prdm15Δ/Δ; Wif1–/–; SPRY1-OE + RSPO1-CM ESCs. Representative images are shown (n = 10).
