Supplementary Figure 2: Prdm15 overexpression does not affect ESC properties.
From: PRDM15 safeguards naive pluripotency by transcriptionally regulating WNT and MAPK–ERK signaling
(a) Prdm15 overexpression (PR15-OE) levels measured by qPCR. (b) Cell cycle analysis by flow cytometry in ESCs upon exogenous expression of Prdm15 or empty vector (EV) control. (c) Colony formation assay for control versus PR15-OE ESCs after 6 d of culture in SL. (d) AP staining on control (EV) versus PR15-OE ESCs; two independent clones are shown. (e) Histological analysis of teratomas formed by control (EV) and PR15-OE ESCs. In both conditions, teratomas contained derivatives of all three germ layers (ectoderm/neural ectoderm in the second panel; mesoderm and endoderm). Representative images are shown (n = 4). One representative experiment with three independent cultures is shown in a–d (n = 2). Center values, mean; error bars, s.d. Student’s t test (two-sided) was used.
