Supplementary Figure 6: ERG recruits prostate master transcription factors to T2E-up elements. | Nature Genetics

Supplementary Figure 6: ERG recruits prostate master transcription factors to T2E-up elements.

From: TMPRSS2–ERG fusion co-opts master transcription factors and activates NOTCH signaling in primary prostate cancer

Supplementary Figure 6

(a) Motif enrichment for selected motifs in cis-regulatory elements that were not significantly different between T2E and non-T2E tumors. (FKH: Forkhead; ARE: androgen response element; ETS: E26 transformation specific; HBOX: homebox). (b) Western blot of ERG, FOXA1, and HOXB13 following depletion of ERG with siRNA in VCaP cells. Vinculin was blotted as a loading control. (siCtl: non-targeting siRNA; siERG: ERG targeting siRNA). (c) ChIP-seq signal plots of average HOXB13 tag density in VCaP cells treated with control non-targeting siRNA or ERG targeting siRNA at HOXB13/ERG co-bound T2E-up elements, (d) HOXB13 bound T2E-up elements not ERG bound, (e) HOXB13 bound T2E-down elements, and (f) HOXB13 peaks that are not T2E-up, T2E down, and not bound by ERG. (g) ChIP-seq plots of average FOXA1 tag density in VCaP cells treated with control non-targeting siRNA or ERG targeting siRNA at FOXA1/ERG co-bound T2E-up elements, (h) FOXA1 bound T2E-up elements not ERG bound, (i) FOXA1 bound T2E-down elements, and (j) FOXA1 peaks that are not T2E-up, T2E down, and not bound by ERG. (k). Co-immunoprecipitation of FOXA1, ERG, and HOXB13 transcription factors in VCaP cells. Arrows indicate expected size of protein on blot. (IP: immunoprecipitation; WB: western blot).

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