Supplementary Figure 5: FIE, AZF1, BPC1 and H3K27me3 ChIP-seq analysis.
From: Cis and trans determinants of epigenetic silencing by Polycomb repressive complex 2 in Arabidopsis
(a,b) Principal component analysis (PCA) of RPM-normalized ChIP and input DNA reads for narrow (a) and broad (b) peak calling. (c) Peak size distribution. (d) Enrichment (P values; converted from the Z-scores of the motif enrichment calculations using a normal distribution) and frequency (%) of PRE cis motifs under FIE-bound peaks. NE: not enriched (P > 0.5). (e) Functional classification of PRC2 (FIE), C1-2iD ZnF (AZF1) and class I BPC (BPC1) peak associated genes. Enrichment of Gene Ontology terms (FDR < 10-5 in at least one of the datasets) for the genes associated with FIE, BPC1 and AZF1 peaks (Q < 10-10). The majority of the significant Gene Ontology (GO) terms of BPC1 and AZF1 targets are also significant GO terms of FIE targets. Enriched GO terms include regulation of transcription, postembryonic development (reproductive development, shoot development, gynoecium development) and hormone response.
