Supplementary Figure 9: Phenotype of simultaneous knockdown of C1-2iD ZnF and class I BPC transcription factors.
From: Cis and trans determinants of epigenetic silencing by Polycomb repressive complex 2 in Arabidopsis
(a) Leaf curling (inset) and early flowering in the wild type (WT), two independent BPCKD ZnFKD TF family knockdown lines, the hypomorph clfR mutant and the null clf-50 mutant. Photographs show plants 6 weeks after germination. Scale bar, 1cm. (b) Quantification of the phenotypes in (a). Box and whisker plot with median (red line, n = 15 plants), upper and lower quartile (box edges), and minima and maxima (whiskers). Letters above boxes indicate significantly different groups (P < 0.05 based on Kruskal–Wallis test with Dunn's post hoc test). (c) Misexpression of Polycomb target genes in the genotypes listed in (a). Expression in the mutant lines is shown relative to that of the wild type. Mean ± s.e.m. from three experiments (red dots). * P < 0.05; ** P < 0.01, NS, not significant (P > 0.05); relative to the wild type, one-tailed unpaired t-test. The two independent BPCKD ZnFKD TF family knockdown lines are not significantly different from each other, NS, not significant (P > 0.05); one-tailed unpaired t-test.
