Supplementary Figure 3: Reintroduction of BAF47 drives genome-wide increases in BAF complex occupancy. | Nature Genetics

Supplementary Figure 3: Reintroduction of BAF47 drives genome-wide increases in BAF complex occupancy.

From: SMARCB1 is required for widespread BAF complex–mediated activation of enhancers and bivalent promoters

Supplementary Figure 3

(a) Venn diagram of BRG1 and BAF155 peaks in (left) empty vector and (right) BAF47 conditions in TTC1240 cells. (b) Correlation plot of log2(fold change) for BRG1 and BAF155 over all BRG1-BAF155 sites in empty or BAF47 conditions in TTC1240 cells. (c-e) Example tracks for BRG1, BAF155, and RNA-seq at (c) LAMB1, (d) FBN1, and (e) HEG1 loci in TTC1240 empty vector and BAF47 conditions. (f) Venn diagram of BAF complex (BRG1-BAF155 overlapping) sites in TTC1240 cells. (g) Distance to closest transcription start site (TSS) for conserved (Empty-BAF47) and gained (BAF47-only) BRG1 and BAF155 sites in TTC1240 cells. (h) Centrimo plots for top four centrally enriched motifs at conserved (Empty-BAF47) BRG1-BAF155 sites in TTC1240 cells. (i-j) Average sequence conservation (PhyloP) in conserved and gained BRG1-BAF155 sites in TTC1240 cells separated by (i) promoter-proximal (≤2kb from TSS) and (j) promoter-distal (>2kb from TSS) sites.

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