Supplementary Figure 5: BAF47 rescue drives genome-wide increases in BAF complex occupancy across MRT and EpS cell lines. | Nature Genetics

Supplementary Figure 5: BAF47 rescue drives genome-wide increases in BAF complex occupancy across MRT and EpS cell lines.

From: SMARCB1 is required for widespread BAF complex–mediated activation of enhancers and bivalent promoters

Supplementary Figure 5

(a) Venn diagram of BRG1 and BAF155 peaks in (left) empty vector and (right) BAF47 conditions in G401. (b) Venn diagram of BAF155 peaks in empty vector and BAF47 conditions in G401 cells. (c) Heatmaps of BRG1 and BAF155 occupancy in G401 empty vector and BAF47 conditions over all BRG1-BAF155 shared sites in the G401+BAF47 condition. (d) Example tracks reflecting BRG1 and BAF155 gain of occupancy at LAMB1 locus. (e) Venn diagram of BRG1 and BAF155 peaks in (left) empty vector and (right) BAF47 conditions in HS-ES-2M. (f) Venn diagram of BAF155 peaks in empty vector and BAF47 conditions in HS-ES-2M cells. (g) Heatmaps of BRG1 and BAF155 occupancy in HS-ES-2M empty vector and BAF47 conditions over all BRG1-BAF155 shared sites in the HS-ES-2M+BAF47 condition. (h) Example tracks reflecting BRG1 and BAF155 gain of occupancy at NRP1 locus. (i) Venn diagram of BRG1 and BAF155 peaks in (left) empty vector and (right) BAF47 conditions in VA-ES-BJ cells. (j) Venn diagram of BAF155 peaks in empty vector and BAF47 conditions in VA-ES-BJ cells. (k) Heatmaps of BRG1 and BAF155 occupancy in VA-ES-BJ empty vector and BAF47 conditions over all BRG1 sites in the VA-ES-BJ+BAF47 condition. (l) Example tracks reflecting BRG1 and BAF155 gain of occupancy at the TGM2 locus. (m-o) Distance to closest transcription start site (TSS) for conserved (Empty-BAF47) and gained (BAF47-only) BRG1 and BAF155 sites in (m) G401, (n) HS-ES-2M, and (o) VA-ES-BJ cells.

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