Supplementary Figure 2: BAF47 contributes to BAF complex affinity on chromatin. | Nature Genetics

Supplementary Figure 2: BAF47 contributes to BAF complex affinity on chromatin.

From: SMARCB1 is required for widespread BAF complex–mediated activation of enhancers and bivalent promoters

Supplementary Figure 2

(a) Schematic of differential salt extraction protocol for determining chromatin affinity of chromatin-binding proteins. (b) Differential salt extraction experiment in G401 empty and BAF47 conditions with western blot analysis for BAF complex subunits, biological replicate of experiment shown in Fig. 1h. (c) Densitometry of BAF155 relative to total. Error bars = mean ± SEM for n=2 biological replicates. (d) Differential salt extraction experiment in empty and BAF47 conditions in G401 cells with western blot analysis for PRC2 subunits, GAPDH, and H3. (e-f) Differential salt extraction experiments in naïve and BAF47-knockout (BAF47Δ/Δ) HEK293T cells with western blot analysis of (e) BAF complex subunits, (f) PRC2 subunits, and TBP. (g) Densitometry of BRG1 in HEK293T naïve and BAF47Δ/Δ conditions relative to total.

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