Supplementary Figure 5: Validation of HiChIP-identified CD69 distal enhancers with CRISPR activation.

CRISPRa validation in Jurkat cells of CD69 distal enhancers. CD69 protein levels are shown for individual sgRNAs tiling H3K27ac HiChIP-identified distal CD69 enhancers relative to the KLRF2 promoter as a locus negative control and a non-targeting negative control.