Supplementary Figure 1: H3K27ac HiChIP enriches enhancer–promoter-associated chromatin contacts.

(a) Schematic of chromatin contacts captured in H3K27ac HiChIP. (b) Loop call overlap for cohesin HiChIP and H3K27ac HiChIP in mES cells. (c) Contact distance distribution for loops that are biased in cohesin versus H3K27ac HiChIP. (d) Proportion of cohesin- and H3K27ac-biased HiChIP loops that have cohesin, CTCF, H3K27ac, and RNA polymerase II binding in at least one loop anchor. (e) Virtual 4C interaction profile of an H3K27ac-biased loop focused at the Malat1 promoter. (f) Virtual 4C interaction profile of a cohesin-biased loop with low transcriptional activity associated.