Supplementary Figure 4: CERES improves the specificity of CRISPR–Cas9 essentiality screens while preserving data in copy number–amplified regions. | Nature Genetics

Supplementary Figure 4: CERES improves the specificity of CRISPR–Cas9 essentiality screens while preserving data in copy number–amplified regions.

From: Computational correction of copy number effect improves specificity of CRISPR–Cas9 essentiality screens in cancer cells

Supplementary Figure 4

(a) The recall common core essential genes at a 5% FDR of nonessential genes is plotted for each cell line before (red) and after (blue) CERES correction for two previously published datasets. (b) The recall at 5% FDR is plotted after correction using a linear model of copy number correction for the Avana dataset. (c) Using a simple filtering scheme removing all genes with a copy number > 4, the total number of genes filtered per cell line is plotted on the left, and the number of genes per cell line with a CERES gene effect < -0.6 on the right. The means are shown with dotted lines.

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