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cDNA microarrays detect activation of a myogenic transcription program by the PAX3-FKHR fusion oncogene

Nature Genetics volume 23, page 55 (1999)Cite this article

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cDNA microarrays allow large-scale analysis of the effects of oncogenic transcription factors on gene expression. Alveolar rhabdomyosarcoms (ARMS) are aggressive soft tissue tumours of striated muscle in which 60% have a reciprocal translocation involving chromosomes 2 and 13, [t(2;13)(q35;q14)]. This results in a novel fusion transcription factor, PAX3-FKHR. Using retroviral transduction we have introduced both PAX3 and PAX3-FKHR into NIH3T3 cells and used a mouse cDNA microarray containing 2,200 elements to investigate their effects on gene expression. Introduction of PAX3-FKHR resulted in increased expression of several genes, including that encoding the myogenic transcription factor myogenin, as well as other muscle-specific genes. In contrast, PAX3 failed to induce these genes and repression of several genes was noted, some of which were also downregulated by PAX3-FKHR. This study demonstrates the potential of cDNA microarrays to elucidate the pathways of malignant transformation by oncogenic transcription factors.

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Authors and Affiliations

  1. NHGRI/NIH, CGB, Bethesda, 20892, Maryland, USA

    Javed Khan, Michael L. Bittner, Lao H. Saal, Alicia J. Faller, Ulrike Teichmann, David O. Azorsa, Gerald C. Gooden, William J. Pavan, Jeffrey M. Trent & Paul S. Meltzer

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  1. Javed Khan
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  2. Michael L. Bittner
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  3. Lao H. Saal
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  4. Alicia J. Faller
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  5. Ulrike Teichmann
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  6. David O. Azorsa
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  7. Gerald C. Gooden
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  8. William J. Pavan
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  9. Jeffrey M. Trent
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  10. Paul S. Meltzer
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Khan, J., Bittner, M., Saal, L. et al. cDNA microarrays detect activation of a myogenic transcription program by the PAX3-FKHR fusion oncogene. Nat Genet 23 (Suppl 3), 55 (1999). https://doi.org/10.1038/14339

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