Supplementary Figure 3: VHL-sufficient and VHL-deficient CD8+ T cell response to acute viral infection.
From: Hypoxia-inducible factors enhance the effector responses of CD8+ T cells to persistent antigen
VHL-sufficient and VHL-deficient CD8+ T cell response to acute viral infection. (a) Wild-type or Vhlfl/fl dLck (VHL-KO) mice were infected with LCMV Armstrong and the CD8+ T cell response in peripheral blood was monitored with MHC class I H-2Db gp33 tetramers to detect LCMV-specific CD8+ T cells. Each line indicates an individual mouse; n=6, representative of two experiments. (b-e), A 1:1 mixture of 104 VHL-sufficient and VHL-deficient P14 CD8+ T cells were transferred to B6 hosts followed by infection with LCMV Armstrong one day later; n=3, error bars indicate s.e.m. (b) Experimental scheme. (c) Frequency of transferred VHL-sufficient and VHL-deficient P14 virus-specific cells in peripheral blood as a % of host CD8+ T cells. (d) Absolute number of VHL-sufficient and - deficient P14 splenocytes on day 8 of infection, * P = 0.0002, (Student's unpaired t-test) (e) KLRG1 expression by VHL-sufficient and VHL-deficient P14 cells on day 7 of infection; n=3, * P = 0.007 (Student's unpaired t-test). Results representative of three independent experiments.
