Supplementary Figure 7: The number of GL7⁺ and BrdU⁺ cells is lower in rapamycin-treated mice than in control mice.

Mice were infected with HKx31virus and received rapamycin or PBS daily. Four and two hours prior to harvest, mice received an injection of BrdU. Spleen and MLN cells were harvested 15 or 20 days after HKx31 infection and stained with antibodies to B220, BrdU, and GL7 to determine (a-b) the number of GL7⁺ B cells (c-d) the number of BrdU⁺ B cells, and (e-f) the percent GL7⁺B cells that are BrdU⁺. Data represent averages ± s.e.m. (n = 5 per group; *P < 0.05 and **P < 0.01, Mann-Whitney U test.