Supplementary Figure 4: ELF4 is involved in TLR-, RLR- and STING-mediated signaling.

(a) 293T cells were transfected with scrambled siRNA, MAVS specific siRNA or ELF4 specific siRNA. 24 h later, cells were infected with SeV or transfected with poly(IC). 24 h later, the according supernatants were transferred to 2fTGH-ISRE-Luc cells. **P<0.01 as determined by Student's t-test. Data were pooled from 3 independent experiments. There are 2 technical replicates within each experiment. (b) Wild type, Sting^GT/GT or Elf4^-/- bone marrow derived macrophages (BMDM) were treated with VSV or PRR ligands, LPS, R848, or transfected with poly(dAdT). The TNFα protein concentration in the cell culture medium was measured by ELISA. Data were pooled from 3 independent experiments. There are 2 technical replicates within each experiment. (c) Wild type, Sting^GT/GT or Elf4^-/- MEF cells were treated with SeV, EMCV, or HSV-1,. The cellular Cxcl10 transcript abundance was quantified by qRT-PCR (normalized to β-actin expression). *P<0.05 and **P<0.01 as determined by Student's t-test. Data were pooled from 3 independent experiments. There are 2 technical replicates within each experiment. (d) 293T cells were transfected with IFNβ-Luc plasmid increasing amounts of scrambled siRNA. MAVS, STING or TRIF specific siRNA as well as ELF4, MAVS, TRIF or STING. **P<0.01 as determined by Student's t-test. Data were pooled from 3 independent experiments. There are 2 technical replicates within each experiment. (e-h). 293T cells were transfected with IFNβ-Luc plasmid or ISRE-Luc plasmid and increasing amounts of scrambled siRNA or ELF4 specific siRNA as well as MAVS (e), TRIF (f) , STING (g) or MyD88 (h). **P<0.01 as determined by Student's t-test. Data were pooled from 3 independent experiments. There are 2 technical replicates within each experiment.