Supplementary Figure 1: ELF4 induces IFNβ and interacts with STING.

(a) QRT-PCR (normalized to human β-actin) analysis of IFNβ mRNA after transfecting the 293T cells with the empty vector (EV) or ELF4 at the indicated times the as well as TBK1. **P<0.01 as determined by Student's t-test. Data were pooled from 3 independent experiments.There are 2 technical replicates within each experiment. (b) 293T cells were transfected with human IFNβ-Luc or murine IFNβ-Luc and increasing amounts of human ELF4 (hELF4) or murine ELF4 (mELF4). *P < 0.05 and **P<0.01 as determined by Student's t-test. Data were pooled from 3 independent experiments. There are 2 technical replicates within each experiment. (c) Supernatants of the cells in Fig.1h were transfered to 2fTGH-ISRE-Luc cells, and assessed using a luciferase assay. *P < 0.05 and **P<0.01 as determined by Student's t-test. Data were pooled from 3 independent experiments. There are 2 technical replicates within each experiment. (d-h) 293 T cells were transfected with the empty vector (EV), ELF4, STK38 or MAVS and IFNa2-Luc, IFNa4-Luc, IFNa8-Luc, IFNλ3-Luc or IL-22-Luc plasmid, and measured by the luciferase assay. *P < 0.05 as determined by Student's t-test. Data were pooled from 3 independent experiments. There are 2 technical replicates within each experiment.