Supplementary Figure 4: Hes1-deficient multipotent fetal liver progenitors cultured with Notch ligands have higher expression of Cebpa mRNA. | Nature Immunology

Supplementary Figure 4: Hes1-deficient multipotent fetal liver progenitors cultured with Notch ligands have higher expression of Cebpa mRNA.

From: T cell development requires constraint of the myeloid regulator C/EBP-α by the Notch target and transcriptional repressor Hes1

Supplementary Figure 4

Flow cytometry and qPCR of OP9-DL4 cultures using FL progenitors (Lin-Kit+Flt3+) sorted from Hes1-/- or Hes1+/+ littermate control embryos (E15.5). After 6 days, Mac1-CD25- cells were sorted for qPCR analysis. Shown are mean mRNA expression levels normalized based on GAPDH levels.

Back to article page