Supplementary Figure 2: Developmental potential of Hes1-deficient progenitors in OP9 and OP9-DL4 cultures.

(a) Flow cytometry of OP9 cultures using FL Lin^-Kit⁺Flt3⁺IL-7Rα^- multipotent progenitors or Lin^-Kit⁺Flt3⁺IL-7Rα⁺ lymphoid progenitors sorted from Hes1^-/- or Hes1^+/+ littermate control embryos (E12.5-13.5). Cultures were harvested after 6 days to assay for the presence of myeloid cells and B cells (b-c) Flow cytometry of OP9 -DL4 cultures using FL Lin^-Kit⁺Flt3⁺IL-7Rα^- multipotent progenitors or Lin^-Kit⁺Flt3⁺IL-7Rα⁺ lymphoid progenitors sorted from Hes1^-/- or Hes1^+/+ littermate control embryos (E12.5-13.5). Cultures were harvested 7 days after plating to assay for the presence of (b) T cells, and (c) myeloid cells. Results are representative of three independent experiments.