Supplementary Figure 5: Vascular integrity is unaffected by S. aureus infection.
From: Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection
(a) Confocal imaging of ear whole mounts from untreated DPE-GFP mice (left panels) or animals infected with S. aureus (WT) (right panels). Basement membranes are delineated by laminin and endothelium by CD31 labeling. (b) Intravital imaging of Evans blue (red; conjugated to BSA) leakage from S. aureus (ΔHla or WT) infected tissue 6h p.i. Adoptively transferred neutrophils (green) and SHG (blue) are also depicted. The images are tiles of 1mmx1mm region of ear tissue. (c) Confocal imaging of Thy-1 stained ear whole mounts from untreated DPE-GFP mice (left panels) or animals infected with S. aureus (WT) (right panels) showing no appreciable loss of Thy-1+ cells.
