Supplementary Figure 3: Phenotypic analysis of PVM.
From: Perivascular macrophages mediate neutrophil recruitment during bacterial skin infection
(a) Flow cytometric analysis of GFP+ and GFP- macrophages from skin of DPE-GFP mice in comparison to red pulp and peritoneal macrophages. (b) Confocal imaging of cremaster muscle showing the localization of PVM (DPE-GFP, green) in relation to pericytes (anti-smooth muscle actin, red) and endothelial cells (CD31, blue). (c) Expression of Csf1r mRNA transcripts in GFP+ macrophages from DPE-GFP mice. Bars represent mean±SD of 3 independent experiments. (d) Comparative mRNA expression of indicated genes in GFP+ vs. GFP- dermal macrophages from DPE-GFP mice. Bars represent mean±SD of 3 independent experiments. (e) Intravital imaging of ears from Csf1r-EGFP mice showing presence of GFP+ PVM (asterisk). Student's two-tailed unpaired t-test was used to determine the P-value. *P<0.05
