Figure 1: IgG1+ and GFP+ cell populations in IgE-GFP reporter mice infected with N. brasiliensis.
From: Addendum: IgE+ memory B cells and plasma cells generated through a germinal-center pathway
(a) Flow cytometry (left and middle) of mesenteric lymph node cells from IgE-GFP reporter mice at day 14 after infection with N. brasiliensis, among cells in the B220+IgD− gate (outlined area at left). Right, quantification of IgG1+GFP− cells (IgG1), IgG1+ GFPhi cells (DP) and IgG1−GFPhi cells (IgE) among B220+IgD− B cells. Each symbol represents an individual measurement (n = 4); small horizontal lines indicate the mean. (b) Flow cytometry (top and middle) of mesenteric lymph node cells from IgE-GFP reporter mice at day 34 after infection with N. brasiliensis, in the GC or memory B cell gate (outlined areas, middle). Bottom, quantification of IgG1+GFP−, IgG1+ GFPhi and IgG1−GFPhi cells (as in a) in the gates above. Each symbol represents an individual measurement (n = 2); small horizontal lines indicate the mean. Numbers adjacent to outlined areas indicate percent cells in each throughout. Data are representative of four experiments (a) or two experiments with cells pooled from three mice (b).
