Supplementary Figure 2: Membrane IgE staining of IgG1+ GFP− (red, IgG1), IgG1+ GFPhi (blue, DP), and IgG1− GFPhi (black, IgE) cells from mesenteric lymph nodes of IgE-GFP reporter mice at 12 days after infection with N. brasiliensis. | Nature Immunology

Supplementary Figure 2: Membrane IgE staining of IgG1+ GFP− (red, IgG1), IgG1+ GFPhi (blue, DP), and IgG1− GFPhi (black, IgE) cells from mesenteric lymph nodes of IgE-GFP reporter mice at 12 days after infection with N. brasiliensis.

From: Addendum: IgE+ memory B cells and plasma cells generated through a germinal-center pathway

Supplementary Figure 2

At this time point, almost all B220+ IgD− cells are germinal center B cells. Membrane IgE staining with an anti-IgE antibody was performed after treatment of cells with an acid buffer to first remove serum IgE bound to the B cells through the low affinity IgE receptor CD23. The graph shows the results of three measurements of the mean fluorescence intensity (MFI) of each cell population, with a line drawn at the mean value of the three measurements. * p=0.01 using a Student's t-test for each pair-wise comparison.

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