Supplementary Figure 1: Gating strategy for CD135 and CD127 expression on DN1a.
From: Two waves of distinct hematopoietic progenitor cells colonize the fetal thymus
(a) Viable Lin- (the lineage cocktail contains CD3, CD4, CD8, NK1.1, CD19, Gr-1, CD25, TER119 and CD11c) CD117+CD44hi cells were separated by CD24 expression into DN1a and DN1b. The upper right panel shows the expression of CD127 and CD135 in DN1a. The CD135 gate was set using DN2 (Lin+CD117+cells) as negative internal control. The bottom histogram shows CD135 expression of DN1aCD135hiCD127+ (in black, open histogram), DN2 (in grey, open histogram), FL CMP (in grey, filled histogram) and FL CLP (in dark grey, filled histogram). (b) CD135 expression in E13 DN2 (in grey, open histogram) compared to newborn (NB) DN2 (in grey, filled histogram), E13 DN1a CD135hi (in black, open histogram) and to NB DN1a CD135hi (dark grey, filled histogram). (c) DN1a CD135hi (black, open histogram), FL CLP (in dark grey, filled histogram), DN2 (in grey open histogram) and FL CMP (in grey, filled histogram). Dot plots are representative of at least twenty-five independent experiments. (d) CD135 expression in NB DN1 subsets (representative of four independent experiments).
