Supplementary Figure 3: CCR9 and CCR7 expression in FB progenitors and ETPs.
From: Two waves of distinct hematopoietic progenitor cells colonize the fetal thymus
(a) relative expression by Q-RT-PCR of CCR9 in CD135+/-CD127- (MPP and MCP), in CD135hiCD127- (LMPP and LMCP), in CD135hiCD127+ CD24+ and CD24lo (CLP and CRLPs). FL (black bars), FB (white bars) and DN1aCD135hiCD127+ (grey bar) (p=0.0003 between FL and FB CD135hi CD127-, p=0.01 between FB CD127+ CD124lo and DN1a) (b) Relative expression of CCR9 in E13 DN1 subsets (DN1aCD135hiCD127+, DN1aCD135lo and DN1b cells) p=0.0001 between CD135hi and CD135lo. AU arbitrary units representing the relative expression to hprt. Error bars represent the mean ± SEM, in one representative experiment. (c) Expression of CCR9 in E17 DN1a subsets (DN1aCD135hi and DN1aCD135lo). (d) Expression of CCR7 in E13 and E17 ETPs (Lin-CD44+CD25-CD117+) compared to DP cells. Results are representative of three independent experiments.
