Supplementary Figure 1: Detailed characterisation of the effect of HDL on TLR-induced cytokine secretion.

a, C57BL/6 mice were injected i.p with 2 mg recombinant HDL or PBS 6 h before subsequent injection with CpG (20 μg) and D-gal (10 mg), 1 h later serum was collected and cytokines were measured (IL-18 n=10 per group, IL-13 n=18 per group). b BMDMs were pre-treated with HDL (either 2 mg/ml or as indicated) for 6 h and stimulated overnight with CpG (100 nM or as indicated) and IL-6 measured in culture supernatants by ELISA. c, Human PBMCs were pre-treated for 6 h with HDL at indicated concentrations and stimulated overnight with LPS (2.5 ng/ml), R848 (100 ng/ml) or P3C (1 μg/ml) and IL-6 secretion measured (c, left), or PBMCs were stimulated with CpG 2336 (A-type) (1 μM) and IFNα production was measured by ELISA (c, right). a, Data are presented as mean values ±S.E.M. b, Data are presented as the mean ±S.D. and are representative of three independent experiments. c, Data is combined from three individual donors and shown as the mean ±S.E.M.